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P12 – Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing

Journal

ChemPhysChem, Volume 9 Issue 17, Pages 2480 - 2485 (Full article)

Authors

Magnus Brändén, Dr. , Sara Dahlin , Fredrik Höök, Prof. Dr.

Organisation

Department of Applied Physics, Chalmers University of Technology, 41296 Göteborg (Sweden), Fax: +46-31-7723134

Abstract

Pucker up! A novel approach enabling direct measurements of biomolecular transfer across lipid bilayer membranes, using surface plasmon resonance, is demonstrated. The figure shows the transfer of sucrose (S) through melittin pores formed in surface-attached liposomes. By measuring the shift in refractive index in the volume enclosed by the liposomes, the sucrose transfer can be time-resolved and quantified.